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Mister Burke's house is a location in Megaton. It is locked with a very hard lock.
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Mister Burke's house is near the Megaton atomic bomb. Enter the house, there are metal boxes to your left side, a desk to your right side. The bed is at the end.
The key to this house cannot be found in-game (in particular, not on Mr. Burke, dead or alive). The only way to obtain it is by console.
There is no karma loss for stealing Mr. Burke's items, or even entering his house, as his own karma status is "very evil".
Why Burke would have a house in a place he plans to destroy is quite odd. The residents say you cant buy a house so why they would let Burke purchase one is also quite odd.